PT  - JOURNAL ARTICLE
AU  - Aykut Elicora
AU  - Serife Tuba Liman
AU  - Betul Arica Yegin
AU  - Asli Gül Akgül
AU  - Hakan Eroglu
AU  - Kursat Yildiz
AU  - Salih Topcu
AU  - Cuneyt Ozer
TI  - THE EFFECT OF LOCALLY APPLIED TGF-BETA3 ON WOUND HEALING AND STENOSIS DEVELOPMENT IN TRACHEAL SURGERY
AID  - 10.4187/respcare.02357
DP  - 2013 Dec 17
TA  - Respiratory Care
PG  - respcare.02357
4099  - http://rc.rcjournal.com/content/early/2013/12/17/respcare.02357.short
4100  - http://rc.rcjournal.com/content/early/2013/12/17/respcare.02357.full
AB  - Background: Tracheal stenosis constitutes one of the most frequently seen problems in thoracic surgery. Although many treatment modalities to prevent fibroblast proliferation, angiogenesis, or inflammation that causes tracheal stenosis have been attempted, an effective method has not yet been found. In this study, a TGF-beta3/chitosan combination was used for that purpose. Methods: A film-shaped slow-releasing preparation containing TGF-beta3 with basal substance of chitosan was made. Thirty albino Wistar rats were divided into three groups. A full-layer vertical incision was made in the anterior side of the trachea of each rat between the 2nd and 5th tracheal rings. The tracheal incision was sutured. Group A was evaluated as the control group. In Group B, a chitosan-based film substance was placed on the incision line. In Group C, a chitosan- TGF-β3 combination slow-release film-coated substance was placed on the incision line. The rats were sacrificed on the thirtieth day, and their tracheas excised by cutting between the lower edge of the thyroid cartilage and the upper edge of the 6th tracheal ring together with the esophagus. Epithelialization, fibroblast proliferation, angiogenesis, inflammation and collagen levels were evaluated histopathologically by the same histopathologist. Results: It was not found that there were statistically significant differences between the three groups. Cold abscesses were observed at the incision area in both the TGFB3/chitosan combination and chitosan groups. These were thought to have formed due to the chitosan. Conclusion: As this was the first experiment in the literature to use this type of TGF-beta3 formulation, it is intended to change the formulation and perform this study again with a different TGF-beta3-chitosan preparation.