PT - JOURNAL ARTICLE AU - Xiaoying Zhang AU - Wei Peng AU - Sheng Zhang AU - Chunzhi Wang AU - Xiyu He AU - Zhimei Zhang AU - Lina Zhu AU - Yan Wang AU - Zhichun Feng TI - MicroRNA Expression Profile in Hyperoxia-Exposed Newborn Mice During the Development of Bronchopulmonary Dysplasia AID - 10.4187/respcare.01032 DP - 2011 Jul 01 TA - Respiratory Care PG - 1009--1015 VI - 56 IP - 7 4099 - http://rc.rcjournal.com/content/56/7/1009.short 4100 - http://rc.rcjournal.com/content/56/7/1009.full AB - BACKGROUND: Bronchopulmonary dysplasia (BPD) is a chronic lung disease of preterm neonates; the underlying pathogenesis is not fully understood. MicroRNAs (length 21–25 nucleotides) are ribonucleic acid (RNA) molecules that have important functions in development, cellular differentiation, apoptosis, proliferation, and migration; very little is known regarding their role in developmental lung diseases. METHODS: We exposed neonatal mice to either room air or 60% oxygen, beginning at birth, and we used microRNA microarray and real-time polymerase chain reaction on lung samples. RESULTS: The hyperoxia-exposed mice developed a lung injury that mimicked human BPD. Fifty-one microRNAs shared similar profiles in the hyperoxia-exposed BPD lungs and the normal lungs, which indicates that those microRNAs might play a protective role during the septation process. In the BPD lungs, compared to the control lungs, 14 microRNAs were up-regulated, and 7 microRNAs were down-regulated, which indicates that these microRNAs might play an important role in the development of BPD. Some of the candidate microRNAs can regulate cell proliferation. CONCLUSIONS: To our knowledge, this study is the first to identify microRNAs associated with BPD development, which provides a clue for further investigation of their function in BPD development.