Absence of cross-reactivity of IgE antibodies from subjects allergic to Hevea brasiliensis latex with a new source of natural rubber latex from guayule (Parthenium argentatum)☆,☆☆,★,★★
Section snippets
Hevea latex
First, Hevea latex was obtained from two sources. J. R. Bugansky, a senior botanist in the Goodyear Tire & Rubber Co., provided nonammoniated latex (NAL) from clones PB260 and GYT577 of H. brasiliensis trees from a plantation in Sumatra. After tapping of the trees, the latex was mixed with two volumes of stabilization buffer (0.1 mol/L NaHCO3, 50% glycerol, 0.3% [wt/vol] sodium azide, and 5 mmol/L cysteine) and transported at ambient temperature to Albany, California, where it was stored at
RESULTS
In the development phase of the IgE anti-guayule latex assay, the guayule protein sorbent was shown to contain bound guayule protein by the binding of the hyperimmunized mouse polyclonal IgG anti-guayule antibodies. A first indication of an absence of cross-reactivity was the observation that guayule latex proteins preincubated with the IgE anti-Hevea latex reference serum produced no competitive inhibition of human IgE anti-Hevea latex binding to the Hevea sorbent. Also, Hevea latex
DISCUSSION
Sensitization to Hevea latex has occurred in children who receive multiple exposures during surgery and in occupationally exposed adults.1, 16, 17 At present, the only mode of treatment is avoidance of direct contact and exposure to airborne latex allergens (found on cornstarch donning powder). For health care workers, this means the personal use of synthetic examination and surgical gloves and the use of powder-free latex or synthetic gloves by coworkers. For individuals sensitized to Hevea
Acknowledgements
We thank Wayne Coates for assistance and advice in large-scale guayule latex production.
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2023, Environmental Technology and InnovationPolyamine flocculants and creaming agent enhance guayule latex processing
2022, Industrial Crops and ProductsCitation Excerpt :Guayule (Parthenium argentatum, Gray) latex is a source of rubber latex which does not cross-react with latex protein allergy and can be used to make high quality latex products (Scholman et al., 1996; Aamir et al., 1996; Cornish and Lytle, 1999). Guayule latex contains less than 1% of the protein in Hevea latex and cannot bind to anti-Hevea protein human IgE antibodies so cannot induce an allergic reaction (Carey et al., 1995; Siler et al., 1996; Hamilton and Cornish, 2010). Therefore, guayule latex is suitable for medical and consumer products where latex allergy may be a safety concern.
Guayule (Parthenium argentatum) resin: A review of chemistry, extraction techniques, and applications
2021, Industrial Crops and ProductsRapid and complete removal of guayule (Parthenium argentatum) leaves by cryodefoliation, and freeze and thaw induction of rubber particle coagulation
2018, Industrial Crops and ProductsCitation Excerpt :In addition, guayule rubber can be extracted in the form of a high quality, low protein latex, which does not cross react with anti-hevea latex protein antibodies. Therefore, unlike hevea latex products, guayule latex products do not pose a risk of immediate Type I allergic reactions in Type I sensitized people (Siler and Cornish, 1994; Siler et al., 1996). In addition, the residual bagasse, post latex extraction, can increase guayule profitability through the production of biofuels, termite-resistant composite boards, soil amendments, and other specialty chemicals (Ray et al., 2010).
High performance waste-derived filler/carbon black reinforced guayule natural rubber composites
2016, Industrial Crops and ProductsCitation Excerpt :Differences in macromolecular structure also affect rubber compounding and product performance (Rensch et al., 1986). GNR proteins do not crossreact with antibodies raised against Hevea latex proteins (Carey et al., 1995; Hamilton and Cornish, 2010; Siler and Cornish, 1995, 1994; Siler et al., 1996). Also, GNR possesses only 1% of the protein in HNR and 90% of these GNR trace proteins is a single protein, a nonallergenic cytochrome P450 oxidase, making GNR particularly attractive for the production of elastomeric medical products.
A sustainability review of domestic rubber from the guayule plant
2015, Industrial Crops and ProductsCitation Excerpt :The first is the widespread occurrence of Type I latex allergy due to the presence of allergenic proteins in Hevea natural rubber (latex) products (Foster and Coffelt, 2005). In the 1990s, guayule was shown to be a source of latex safe for people with Type I latex allergy, and contained the necessary elasticity, which is not provided by synthetic materials (Siler et al., 1996), and could easily act as a replacement for Hevea in medical and personal products (Nakayama, 2005). The second reason is due to significant increases in guayule rubber yields with improved agronomic practices (Foster and Coffelt, 2005).
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From aUnited States Department of Agriculture–Agricultural Research Service, Western Regional Research Center, Albany; and b The Johns Hopkins University School of Medicine, Baltimore.
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Supported in part by National Institutes of Health grant U01-AI-31867 and by United States Department of Agriculture–Cooperative State Research Service grant 93-COOP-2-9525.
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Reprint requests: Deborah J. Siler, PhD, USDA-ARS Western Regional Research Center, 800 Buchanan St., Albany, CA 94710.
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