Regular Article
Assay of Coenzyme Q10 in Plasma by a Single Dilution Step

https://doi.org/10.1006/abio.2002.5653Get rights and content

Abstract

A new method is described for determining coenzyme Q10 (CoQ10) in plasma. The method is based on oxidation of CoQ10 in the sample by treating it with para-benzoquinone followed by extraction with 1-propanol and direct injection into the HPLC apparatus. This method achieves a linear detector response for peak area measurements over the concentration range of 0.05–3.47 μM. Diode array analysis of the peak was consistent with CoQ10 spectrum. Supplementation of the samples with known amounts of CoQ10 yielded a quantitative recovery of 96–98.5%; the method showed a level of quantitation of 1.23 nmol per HPLC injection (200 μl of propanol extract containing 33.3 μl of plasma). A correlation of r = 0.99 (P < 0.0001) was found with a reference electrochemical detection method. Within run precision showed a CV% of 1.6 for samples approaching normal values (1.02 μM). Day-to-day precision was also close to 2%.

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