Chest
Laboratory and Animal InvestigationsEarly Treatment With Pentoxifylline Reduces Lung Injury Induced by Acid Aspiration in Rats
Section snippets
Anesthesia, Fluid Balance, Monitoring
The local Bioethics Committee approved the experimental protocol for this study. Twenty-four, adult, male Sprague-Dawley rats weighing 280 to 320 g (Charles River; Sulzfeld, Germany) were cared for and handled according to the guidelines of the German National Institute of Health. On arrival at our facility, all rats received food and water ad libitum. Anesthesia was induced in a 2-L container and maintained by inhalation of an inspired concentration of 2.5% sevoflurane, tracheostomy was
Blood Gas Analysis
The Pao2 values and arterial gas analyses are summarized in Figure 1. There were no differences between the groups in the baseline measurements 15 min and immediately prior to tracheal acid instillation. As expected, the Pao2 showed an initial decline in both groups 30 min after acid instillation. At this time point, the Pao2 did not differ significantly between groups, although the placebo group had a mean Pao2 15 mm Hg lower than the pentoxifylline-treated animals. While Pao2 almost returned
Discussion
Although several experimental studies1415 have demonstrated that pentoxifylline has beneficial effects on lung inflammation and may result in decreased lung injury, the effect of early treatment on pulmonary function and hemodynamic parameters after an aspiration event has not been previously reported. Aspiration of gastric components leads to activation of macrophages and release of proinflammatory cytokines such as TNF-α, which is followed by neutrophil infiltration into the interstitial and
Conclusions
Acid aspiration impairs gas exchange and induces hypotension. These data suggest that early treatment with pentoxifylline effectively improves oxygenation, markedly stabilizes systemic hemodynamics, and results in better survival after 6 h in a model of acid aspiration-induced lung injury.
ACKNOWLEDGMENT
The authors thank David Tracey, PhD, Department of Anatomy, University of New South Wales, Sydney, Australia for helpful comments regarding this manuscript.
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