The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We have
recently demonstrated the presence of about six cycling Lgr5 + stem cells at the bottoms of …

BACKGROUND & AIMS: We previously established long-term culture conditions under which
single crypts or stem cells derived from mouse small intestine expand over long periods. …

Human colorectal tumors bear recurrent mutations in genes encoding proteins operative in
the WNT, MAPK, TGF-β, TP53 and PI3K pathways 1 , 2 . Although these pathways influence …

Homeostasis of self-renewing small intestinal crypts results from neutral competition between
Lgr5 stem cells, which are small cycling cells located at crypt bottoms 1 , 2 . Lgr5 stem cells …

The study of gastric epithelial homeostasis and cancer has been hampered by the lack of
stem cell markers and in vitro culture methods. The Wnt target gene Lgr5 marks stem cells in …

The gradual accumulation of genetic mutations in human adult stem cells (ASCs) during life
is associated with various age-related diseases, including cancer 1 , 2 . Extreme variation in …

Recent examples have highlighted how stem cells have the capability to initiate morphogenesis
in vitro; that is, to generate complex structures in culture that closely parallel their in vivo …

Colorectal tumor is a heterogeneous disease, with varying clinical presentation and
prognosis in patients. To establish a platform encompassing this diversity, we generated 55 …

Adult stem-cell therapy holds promise for the treatment of gastrointestinal diseases. Here
we describe methods for long-term expansion of colonic stem cells positive for leucine-rich …

Intestinal stem cells, characterized by high Lgr5 expression, reside between Paneth cells at
the small intestinal crypt base and divide every day. We have carried out fate mapping of …